The expressed recombinant protein represented the maj... We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchin Paracentrotus lividus. Vincenzo has 8 jobs listed on their profile. of Northern blots and ofin vivo labeling experiments we provide evidence for the following facts: 1) the rate of rRNA synthesis per embryonic nucleus in 36. A spectrophotometric study of methylene blue dimerization in aqueous solutions of some monohydric alcohols, Solvent isotope effect in the monomer?dimer equilibrium of methylene blue, Effects of Organic Solvents on the Conformational and Functional Stability of Some Biological Macromolecules, Effect of some monohydric alcohols on the functional stability of bovine liver ?-galactosidase, Thermal denaturation of B. subtilis DNA in H 2 O and D 2 O observed by electron microscopy, 266 Comparison of Allergen-Induced Late Inflammatory Reactions in the Nose and in the Skin in House Dust Mite-Allergic Patients with or without Asthma. Publications. Species belonging to this genus induce IgE responses in approximately 10 million people. The Getty Images design is a trademark of Getty Images. We report on experiments showing that the presence of some monohydric alcohols in the solution medium perturbs the kinetics of bovine liver β-galactosidase. Izzo Caffè was founded near the Vesuvius in 1979 by Mr. Vincenzo Izzo as an artisan-based coffee roastery, which have been striving with passion to produce real Neapolitan espresso for over 25 years. Par j 2.0101, a major allergen of the Parietaria judaica pollen, was expressed in E. coli, purified to homogeneity and fully characterised both at the structural and the functional level. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies. A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Aware of the progresses achieved so far, and steadily committed to continuous and constant improvement, we pride ourselves today on playing an important role in the complex scenario of coffee business, especially thanks to the dynamism of our ideas. In this paper the use of voltage gradient gel electrophoresis (VGGE) in the electrophoretic analysis of apoptotic DNAs is described. In the present work we studied the initial part of thermal denaturation curves of B. subtilis DNA in normal and heavy water, observing, by electron microscopy, the “opening” of DNA as a function of temperature. The clone contained an insert of 622 nucleotides with an open reading frame of 133 amino acids (aa) and a putative signal peptide of 31 aa giving a deduced mature... A gene for the Paracentrotus lividus ribosomal protein S24, called P1-S24, has been isolated and sequenced. {{familyColorButtonText(colorFamily.name)}}, View {{carousel.total_number_of_results}} results. results support the hypothesis that D2O plays a stabilizing role on strands separation during thermal denaturation of DNA. Computer-aide... As it is well known, solvent perturbation due to the presence of organic solvent in the solution medium affects the conformational equilibria of biological macromolecules1,2,3. Network. Il clan degli Izzo, come raccontato anche dalla gieffina Myriam Catania, è molto unito; una grande famiglia che ama riunirsi e passare del tempo insieme. This repository is populated with tens of thousands of assets and should be your first stop for asset selection. Publications 36. the sea urchinP. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas plate placed slantwise with respect to the gel surface plane, subm... By the use of labeled DNA probes which selectively hybridize to non mature ribosomal RNA precursors and by the combined use Sequence analysis shows a high percentage (90%) identity with the corresponding gene of Strongylocentrotus purpuratus. Vincenzo Izzo. The same approach was used to measure the copy number of the genes involved in ribosome biogenesis. © 2008-2020 ResearchGate GmbH. A DNA-containing gel slice is placed on a Parafilm sheet in the center of a circular (positive) electrode and covered with a drop of buffer, while a linear (negative) electrode is placed on the top of the gel and driven about 1 mm... Blot-hybridizations of the sea urchin Paracentrotus lividus genomic DNA with ribosomal DNA (rDNA) probes revealed individual variations in the length and in the sequence of the non-transcribed spacer (NTS) region. Izzo Caffè was founded near the Vesuvius in 1979 by Mr. Vincenzo Izzo as an artisan-based coffee roastery, which have been striving with passion to produce real Neapolitan espresso for over 25 years. Today the Company are offering a series of activities related to each other, which pursue the Company's aim to provide the market not only with a wide range of products but also with services tending to guarantee maximum customer satisfaction. If you decide to continue browsing we consider that you accept their use. Biography. Only verified researchers can join ResearchGate and send messages to other members. GRUPPO IZZO Srl - Italy - 03012 Anagni (FR) Via Morolense, Km 2 Tel +39 0775 774036 - Fax +39 0775 768524 - P.IVA 03043961212 - Credits. Caffè Izzo awarded COFFEE OF THE YEAR 2016 Izzo Caffè - Our history . Contact. Twenty-nine single sera from Pj-allergic subjects were test... We built a modified version of voltage gradient gel electrophoresis system to correct distortions in nucleic acids electrophoretic migration patterns occurring at the edges of the gel when the original voltage gradient apparatus is used. The IgE-binding regions of the Parietaria judaica (Pj) pollen major allergen rPar j 2 were investigated. Ribosomal protein P1-S24 consists 130 amino acids and has a molecular weight of 14869 Da. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S... Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. Sorry, you need to be a researcher to join ResearchGate. About. Among the services the customers can benefit from, the Company have recently devised a formula for the franchised Izzo Coffee Shops, in cooperation with their business partners; cafeterias to which a series of promotional and commercial initiatives based on exclusive Izzo Products are bound. Both clones contain a putative signa... A clone (P2) coding for an allergen of Parietaria judaica (Pj) pollen has been isolated and sequenced from a cDNA library in lambda ZAP using a pool of 23 sera from Pj-allergic patients.
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